Gro-Seq Plant ChIP Kit (48 rxns)

Protein-DNA interaction play a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of genes that effect homeostasis, development, and adaptation. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for identifying direct genomewide associations between specific regulatory proteins and their target genes. Unlike other methods such as EMASA, DNA microarrays, and report gene assays, which analyze direct interactions between protein and DNA in vitro, ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells.

This ChIP kit includeds all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly, this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting materials can include various plant tissue (flowers, leaves, young seedlings). In general, the input amount should be from 20 to 50 mg of plant tissue for each reaction.