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Gro-Seq Plant MeDIP Kit (48 rxns)

Product Overview

The Gro-Seq Plant MeDIP Kit is a complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format. The method, methylated DNA immunoprecipitation (MeDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The highly sensitive and specific format of the kit can use DNA isolated from various species. The methylated DNA that is enriched with this kit can be used for various downstream applications including qualitative and quantitative PCR (MeDIP-PCR), microarray (MeDIP-chip) and especially sequencing (MeDIP-seq). The kit has the following advantages and features:

  • Extremely fast and convenient protocol with a total procedure time (from input sample to ready-to-use methylated DNA) of less than 3 hours, which includes a minimal handling time of less than 20 minutes.
  • Optimized buffers and protocol allow minimal background by overcoming the weaknesses that cause non-specific enrichment.
  • A highly specific 5-mC monoclonal antibody included in the kit can strongly bind both single and double stranded DNA fragments containing 2 or more 5-mCs which enables highly sensitive enrichment of methylated DNA with >99% specificity. 
  • Flexible 96 strip-well microplate format makes the assay very easy to handle: manual method with one reaction at a time or high throughput method with 24-48 reactions at a time.
  • Spin columns and collection tubes conveniently included for a DNA purification step.
  • Low DNA input requirement as low as 50 ng (10,000 cells) per reaction.
  • High reproducibility using pre-optimized MeDIP conditions.
  • Compatible with various downstream analysis workflows including MeDIP-PCR and MeDIP-chip, and specifically for MeDIP-seq.

Gro-Seq Plant MeDIP Kit (48 rxns)

  • View and download product SDS here.

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